Collagen synthesis: a disulfide-linked collagen precursor in chick bone.
نویسندگان
چکیده
Chick calvaria labeled in vitro contain a triple-stranded collagen precursor that contains two pro alpha1 and one pro alpha2 chains. All chains contain cystine and are linked by S-S bonds. Ultracentrifugal analysis was used to measure the relative size of the disulfide-linked units. It is proposed that the S-S links help to determine the correct alignment of the triple-stranded structure.
منابع مشابه
Characterization of type III procollagen from chick embryo blood vessels.
The precursors of collagen types I and III were extracted from radioactively labeled chick embryo blood vessels. Procollagen III was found to consist of three identical proa III chains, each containing a large amino and a large carboxyl propeptide. Interchain disulfide bridges occur at three locations: between the amino propeptides, the carboxyl propeptides, and within the vertebrate collagenas...
متن کاملSynthesis of type III collagen by fibroblasts from the embryonic chick cornea
Synthesis of collagen types I, II, III, and IV in cells from the embryonic chick cornea was studied using specific antibodies and immunofluorescence. Synthesis of radioactively labeled collagen types I and III was followed by fluorographic detection of cyanogen bromide peptides on polyacrylamide slab gels and by carboxymethylcellulose chromatography followed by disc gel electrophoresis. Type II...
متن کاملCollagen Metabolism in the Normal and Lathyritic Chick
1. Radioisotope incorporation studies of normal and lathyritic chick embryo bone collagen do not demonstrate any interference by lathyrism with collagen synthesis or fibril formation. 2. The results indicate that a portion of the extractable collagen from lathyritic chick embryo bone represents newly synthesized protein. Evidence from a double labeling experiment and from analysis of isotope fl...
متن کاملVitamin A and bone formation. Effect of an excess of retinol on bone collagen synthesis in vitro.
The influence of an excess of retinol on bone formation was studied by using cultures of embryonic-chick calvaria. Retinol decreased collagen synthesis in a dose-dependent manner, non-collagenous protein synthesis being relatively unaffected. Collagen synthesis was significantly inhibited after 24 h of culture with retinol and was progressively decreased, compared with control cultures containi...
متن کاملNative cross-links in collagen fibrils induce resistance to human synovial collagenase.
A model system consisting of highly purified lysyl oxidase and reconstituted lathyritic chick bone collagen fibrils was used to study the effect of collagen cross-linking on collagen degradation by mammalian collagenase. The results indicate that synthesis of approx. 0.1 Schiff-base cross-link per collagen molecule results in a 2--3-fold resistance to human synovial collagenase when compared wi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 70 10 شماره
صفحات -
تاریخ انتشار 1973